The assay is run in two main steps, the kinase reaction step followed by the detection step with HTRF reagents (Fig. 1). The phosphorylation of the biotin-tagged generic peptide is initiated by ...

The Pim kinase family of serine/threonine kinases is composed of three isoforms, Pim-1, Pim-2 and Pim-3, which have an important role in tumor progression. Pim kinases suppress apoptosis and ...