A newly developed luciferase-based reporter can detect ... While designing this reporter, the researchers took a page from a fusion protein called MalF-LacZ, derived from Escherichia coli bacteria.

We subcloned the cDNA encoding Factor IX into the same vector used to express luciferase protein. We transiently transfected CHO cells with 3:6 (plasmid DNA/L-PEI) polyplex ratio. We obtained ...

He extracted two components from beetle abdomens, naming the substrate luciferine and the enzyme responsible for the reaction luciferase. 2 Not all bioluminescent marine organism systems can be used ...

The target protein is expressed as a NanoLuc ® luciferase fusion. A cell-permeable fluorescent NanoBRET ™ tracer is then added, which reversibly binds to the target protein. NanoBRET ™ TE is ...

The fusion protein MalF-LacZ, which is derived from ... natural processes and created a reporter molecule based on firefly luciferase (FLuc) that functions similarly. When oxygen, adenosine ...