Reporter gene expression. Plasmid DNA (35 μg in 25 μl PBS) encoding firefly luciferase (pCMV-Luc; Aldevron, Fargo, North Dakota) was administered as above. Then, 24 h after delivery, full ...

Using luminescence live-imaging with luciferase as a reporter, the research team observed that NSP1 gene expression, rapidly induced in response to rhizobia and essential for the infection process ...