The cytoplasmic domain of Sso1 and truncation constructs were dialyzed against 20 mM sodium phosphate buffer, pH 6.5, containing 50 mM NaCl. Protein concentrations were determined by internally ...

To improve HPLC resolution after catechin extraction from a commercial green tea bag, a 10 mM (pH 2.5) sodium phosphate buffer for the aqueous mobile phase and acetonitrile as an organic eluent ...

1| Wash thirty 10-cm plates of the appropriate cells (a total of ∼6 × 10 7 cells) in phosphate-buffered saline ... CAUTION), 0.2 mM sodium orthovanadate, 100 mM sodium fluorine ( CAUTION)).