Thiazole orange probe design and synthesis. Detection and imaging of cellular DNA and RNA are increasingly important fields of study21,22 and fluorogenic intercalators are potentially valuable in this context. Hence we undertook investigations on the fluorescent and duplex stabilising properties of a number of TO probe designs. For synthetic ...

2009年1月15日 · A series of Thiazole Orange (TO) derivatives were synthesized and modified. TO derivatives modified by folic acid were also studied and their preliminary labeling breast cancer cells was carried out.

2019年4月17日 · Thiazole orange (TO) is an unsymmetrical cyanine dye which can be conjugated to oligonucleotides (ONs) to create fluorogenic hybridisation probes. 1–3 TO becomes highly fluorescent upon binding to nucleic acids due to restriction of rotation about the methine bridge, hence TO–ON conjugates have been used for the detection of target nucleic ...

2012年4月6日 · Thiazole Orange is a fluorescent dye commonly used in chemistry as a 'light-up probe' in assays such as real-time PCR. It exhibits a significant increase in fluorescence intensity when bound to a complementary nucleic acid, making it a valuable tool for monitoring amplification reactions.

2011年5月2日 · The title compound of Thiazole Orange derivative was synthesized by the reaction of benzothiazolium and 4-methyl quinoline salts, which was determined by 1HNMR and MS. A crystalline hydrate of thiazole orange derivative was obtained when the crystal formed and characterized by single-crystal X-ray diffraction.

2005年3月15日 · Thiazole orange dyes were derivatized with ethylene glycol linkers of various lengths, and were covalently linked to the 5′ end of the oligonucleotides after solid-phase synthesis. The labeled oligonucleotides exhibited enhanced fluorescence upon hybridization to complementary DNA sequences at the surfaces of optical fibers, providing for a ...

presents a comprehensive study of six Thiazole-Orange-based fluorescent DNA-binding dyes: SYBR Safe, SYBR Green, Pico Green, SYTO-16, SYTO-9, and the benzothiazole-based analogue (TOPhBu) of the latter. The selected DNA markers were synthesized at a 10-milligram scale and characterised spectro-scopically to quantify their fluorescence ...

2009年1月15日 · A series of Thiazole Orange (TO) derivatives were synthesized and modified by introducing different substitutional groups on benzothiazole and 4-methylquinoline All the TO derivatives were confirmed by (1)HNMR and MS. TO derivative bearing NH(2)- was modified by folic acid and used to label breast c …

Here, we introduce a TO-containing acyclic nucleotide, which is coupled during automated oligonucleotide synthesis and provides for the desired fluorescence-up properties. The study reveals the conjugation mode as the most important issue.

2019年6月3日 · We have synthesised a range of thiazole orange (TO) functionalised oligonucleotides for nucleic acid detection in which TO is attached to the nucleobase or sugar of thymidine. The properties of duplexes between TO-probes and their DNA and RNA targets strongly depend on the length of the linker between TO and Chemical Biology in OBC