2020年3月6日 · We set out to establish conditions for mRNA transfection in hard-to-transfect primary human monocytes and monocyte-derived macrophages due to the great potential of gene expression from in vitro...

2023年8月2日 · In this study, we synthesized carbon nanodots (CNDs) as an efficient mRNA delivery vehicle via a one-step microwave-assisted method. CNDs easily formed complexes with mRNA molecules by electrostatic interactions, and the gene delivery performance of CNDs was highly effective in hard-to-transfect cells.

Messenger RNA (mRNA) transfection can be performed both in vitro and in vivo using a variety of methods (e.g., cationic lipid-mediated delivery or electroporation). Of note, ionizable lipid nanoparticles (LNPs) have become a more desirable mRNA delivery mechanism in clinical research as LNPs have demonstrated reduced toxicity compared to ...

Transfection of RNA is an offshoot of classic transfection technologies for introducing RNA into cells. The purpose of RNA transfection is similar to that of plasmid transfection. mRNA is introduced into cells to express the encoded protein, and study gene function and regulation. siRNA is used for RNAi studies that examine the effects of gene ...

The prepared mRNA samples were stored at −80 °C until use in subsequent transfection or injection experiments, to maintain RNA integrity over time. ... 4.4. mRNA transfection. 2 × 10 5 293 T cells were plated in 24-well plates. When cells reached a confluence of 60 %, they were transfected with mRNA (0.8 µg/well) using Lipofectamine ...

RNA 转染是将 RNA 导入细胞的经典转染技术的分支。 RNA 转染的目的与质粒转染相似。 将 mRNA 导入细胞旨在表达编码蛋白以及研究基因功能和调控。 siRNA 用于评估基因敲低影响的 RNAi 研究。 这两种方法的一个主要区别是 RNA 只能进行瞬时转染。 下图描绘了 siRNA 设计和合成后的 RNAi 实验工作流程。 在进行 RNAi 实验时，请确保您已具备： 图1：siRNA 设计和合成后的 RNAi 工作流程. RNA 寡核苷酸在处理过程中易于被引入的外源性核糖核酸酶降解。 处理 …

2024年12月19日 · In this study, the mRNA delivery efficacy of N1-501 across 18 cell lines is quantitatively assessed using eGFP expression assays, demonstrating versatile and robust performance across cell types. Importantly, systemic in vivo delivery of mRNA using N1-501 resulted in remarkable spleen tropism.

2021年11月1日 · We designed a novel microfluidic device that allows for efficient mRNA delivery via volume exchange for convective transfection (VECT). In the device, cells flow through a ridged channel that...

The combination of point-directed transfection and mRNA transfection is a new way of studying the function of genes and gene products. However, each method has its own advantages and disadvantages so the optimum method depends on experimental design and objective.

Messenger RNA (mRNA) electroporation is a powerful tool for transient genetic modification of cells. This non-viral method of genetic engineering has been widely used in immunotherapy. Electroporation allows fine-tuning of transfection protocols for each cell type as well as introduction of multiple protein-coding mRNAs at once.