Diethyl pyrocarbonate (DEPC), also called diethyl dicarbonate (IUPAC name), is used in the laboratory to inactivate RNase enzymes in water and on laboratory utensils. It does so by the covalent modification of histidine (most strongly), lysine , cysteine , and tyrosine residues.

Diethyl pyrocarbonate is the diethyl ester of dicarbonic acid. It is functionally related to a dicarbonic acid. Preservative for wines, soft drinks, and fruit juices and a gentle esterifying agent. C6H10O5. Computed by PubChem 2.2 (PubChem release 2021.10.14) Viscous liquid with a …

Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases. Inactivates RNase in solution at about 0.1% (v/v), thus protecting RNA against degradation. Choose from one of the most recent versions: Don't see the Right Version?

Diethyl Pyrocarbonate (DEPC) is a chemical use to inactivate RNase enzymes and is sensitive to moisture and pH. It decomposes at 155°C, to ethanol and carbon dioxide in aqueous solution. DEPC is also sensitive to ammonia, which causes decomposition to …

Diethyl Pyrocarbonate (DEP) in 2× SSC: Acetic Acid. Prepare 2× SSC : acetic acid first by making an 800 ml solution of 2× SSC and then slowly adding acetic acid until the pH = 3.50. Then add 160 μl of DEP (Sigma D-5758). DEP should be purchased in small …

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Diethyl Pyrocarbonate UNII LMR3LZG146 Molecular formula C 6 H 10 O 5 Molecular weight 162.14 Percent composition C 44.45%, H 6.22%, O 49.34% Standard InChI InChI=1S/C6H10O5/c1-3-9-5(7)11-6(8)10-4-2/h3-4H2,1-2H3 Standard InChIKey FFYPMLJYZAEMQB-UHFFFAOYSA-N

Diethyl pyrocarbonate (DEP) is a bactericidal agent and decomposes in water to CO 2 and ethanol. DEP inhibits enzymes such as DNase and RNase without affecting nucleic acids. It is useful for the preparation of mRNA from tissues.

Considering the broad distribution of the RNases and their great stability, their inactivation is one of the largest problems related to RNA. The most common method used to free solutions from RNase is to treat them with diethyl carbonate. To do so, a 1/200 volume of diethyl pyrocarbonate (DEPC) is added and mixed until the DEPC beads have ...

2024年10月29日 · diethyl pyrocarbonate-- prohibited other names: ♦ diethyl pyrocarbonate ♦ ethoxyformic acid anhydride ♦ diethyl dicarbonate ♦ diethyl oxydiformate ♦ depc ♦ dicarbonic acid, diethyl ester ♦ formic acid, oxydi-, diethyl ester used for* † (technical effect): dough strengthener