2024年2月15日 · Tissue-specific overexpression of systemic RNAi components reduces lifespan in C. elegans. Lifespan reduction interacts with components of systemic RNAi and microRNA …

Here, we demonstrate that overexpressed shRNAs can saturate the activity of endogenous Exportin 5, a factor required for nuclear export of both shRNAs and pre-miRNAs. While shRNA …

Here, we report a set of versatile tools and widely applicable strategies for transient or stable Ago2 co-expression, which overcome these concerns. Specifically, we engineered plasmids …

2024年10月9日 · 本专题主要介绍RNA干扰技术，RNAi是指一些小的与靶基因序列同源的双链RNA (dsRNA) 可以高效、特异性地阻断体内特定基因表达，促使mRNA降解，诱使细胞表现出特定 …

2024年2月15日 · Tissue-specific overexpression of systemic RNAi components reduces lifespan in C. elegans. Lifespan reduction interacts with components of systemic RNAi and microRNA …

2018年12月30日 · In this study, after bioinformatics analysis of TwIDI, we constructed overexpression and RNAi constructs and introduced them into Tripterygium wilfordii …

For the past five years, evidence has accumulated that vector-mediated robust RNA interference (RNAi) expression can trigger severe side effects in small and large animals, from cytotoxicity …

2016年8月3日 · Specifically, we show that distinct overexpression of Argonaute coding sequences cannot be explained by mRNA degradation in the cytoplasm or nucleus, and exhibits on …

2018年12月30日 · The expression of TwIDI was increased to 157% of the control group (empty vector) in the overexpression group, and was reduced to 71% of the control group in the RNAi …

2021年11月23日 · Here we report that the rice osa-miR162a can mediate cross-kingdom RNA interference (RNAi) by targeting the NlTOR (Target of rapamycin) gene of BPH that regulates …